- #Flamingo nxt 5 bitmap mapping update
- #Flamingo nxt 5 bitmap mapping manual
- #Flamingo nxt 5 bitmap mapping software
One-click transition from tubes to plates and vice versa no disassembly, no additional QC, no reboot required for conversion between plates and tubes Hierarchical gating with the ability to derive gatesĭocumented in FCS files and maintained upon importĬreate from existing experiments-instrument settings, workspaces, run protocols, heat map settings, and compensation settings optimized and defined previously Up to 4 individual thresholds with user option to apply Boolean logic Set up for definition of plate layout screening view for analysis for tubes and plates Stops automated run to preserve sample integrity
#Flamingo nxt 5 bitmap mapping update
Live update of statistics during acquisition of events up to 35,000 events/secĬomparative analysis between samples 3D viewĭirect concentration measurement without use of counting beads
#Flamingo nxt 5 bitmap mapping software
Precise flow rate control via software no hardware adjustments
#Flamingo nxt 5 bitmap mapping manual
Up to 6,000 images/second depending on image size and event rateįull matrix-automated and manual modes, on-plot compensation tools for fine adjustment use of tubes and wells 100083194), and Attune Focusing Fluid (Cat. Measured area, height and width pulse for all detectorsĬan be configured for violet side scatter to better resolve particles from noiseĠ.2 μm on side scatter using submicron bead calibration kit from Bangs Laboratories-0.1 μm on side scatter under following conditions: Using an Attune NxT Flow Cytometer with standard 0.5 mm blocking configuration, an Invitrogen Attune NxT 488/10 Filter (Cat. PMT with default 488/10 nm bandpass filter optional 405/10+OD2 nm bandpass filter Photodiode detector with 488/10 nm bandpass filter Optimized to resolve lymphocytes, monocytes, and granulocytes in lysed whole blood Up to 35,000 events/sec, 34 parameters, based on a 10% coincidence rate per Poisson statisticsĪble to discriminate platelets from noise ≤80 molecules of equivalent soluble fluorochrome (MESF) for FITC, ≤30 MESF for PE, ≤70 MESF for APCĬV <3% for the singlet peak of propidium iodide–stained chicken erythrocyte nuclei (CEN) ≤15 min start-up and shutdown-deep clean, sanitize, and debubble modes Positive-displacement syringe pump for volumetric analysisĪccommodates tubes from 17 x 100 mm to 8.5 x 45 mmġ.8 L focusing fluid tank, 1.8 L waste tank, 175 mL shutdown solution tank, and 175 mL wash solution tank Quartz cuvette gel coupled to 1.2 numerical aperture (NA) collection lens, 200 x 200 μm Instant “on/off” reduces usage and/or aging by 10x only turns on when acquiring samples reports hours of usageįlat-top laser specified at the flow cellĬoefficient of variation (CV) <3% over the width of the flat-top laser No warm-up delay fiber isn’t affected by “on/off” Up to 14 color channels with wavelength-tuned photomultiplier tubes (PMTs) user-changeable, keyed filtersįixed alignment with prealigned welded fiber no user maintenance required Optimized excitation for minimized stray laser-line noise and losses to reflectionġ0 x 50 μm flat-top laser providing robust alignment * Amount of measured usable laser power after light has gone through the beam optics and shaping filters.
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